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The pretreated cells were digested with EDTA-free trypsin and washed twice with PBS, the remaining operations were carried out according to the instructions of the S0053, S0033S and S0131S (Beyotime Biotechnology, Shanghai, China) kits

[DOI] [PMC free article] [PubMed] [Google Scholar] 14.Henter ID, Park LT, Zarate CA Jr

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These results reinforce the role of GCLC in regulating not only GSH synthesis but also mitochondrial integrity and cell viability under oxidative stress [34,35,36]